Optimizing alkaline lysis for DNA plasmid recovery

Optimization of the alkaline lysis (P2) and neutralization (N3) steps in the recovery of DNA plasmids was pursued. Experiments were conducted at the test-tube and 5-litre scales with 3 kb (pUC18) and 20 kb (pQR150) plasmids. The scale and degree of mixing/shear did not affect the optimum yield of su...

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Journal Title: Biotechnology and Applied Biochemistry Vol. 37; no. 3; pp. 235 - 244
Authors: W.J. Kelly, M. Clemson
Format: Article
Published: Jun 2003