Expression and isolation of the ankyrin repeat domains of TRPV1 and TRVP2

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Description: Transient Receptor Potential (TRP) channels are sensory transducers for unicellular and multicellular organisms. TRPV1 and TRPV2 are nociceptors and heat sensors. They are involved in anything from itch and asthma to chronic pain and noxious heat. TRPV1 is activated by heat (≥43°C), low pH (≤5.9), and capsaicin. TRPV2 is activated by noxious heat (>53°C), 2-aminoethoxydiphenyl borate (2-APB), growth factors, and osmotic stress. There is evidence that the ankyrin repeat domain located on the N-terminal of each of these isoforms is involved in their activation. The purpose of this research was to express and isolate the ankyrin repeat domain of each isoform. The ankyrin repeat domain of Homo sapiens TRPV1, which was the primary target for the research, was expressed and found to be soluble in water. The ankyrin repeat domain of Rattus norvegicus TRPV2, which was our control, was amplified and subcloned, but it was not expressed successfully.
Language: English
Format: Degree Work